ABSTRACT
OBJECTIVE: MTA1 has been identified as a metastasis-promiting gene, and its gene expression is correlated with invasion and metastasis in several cancers. We examined MTA1 expression levels in epithelial ovarian neoplasm. METHODS: Expression of MTA1 was evaluated by immunohistochemistry and tissue array in 53 benign tumors, 27 borderline tumors and 68 malignant tumors. The data was analyzed in reference to various clinicopathological parameters. RESULTS: Increased expression of MTA1 was significantly correlated with histologic grade and FIGO stage. There was no relationship between MTA1 expression and age, histologic type, tumor size. CONCLUSION: These results suggest that MTA1 is closely related to invasiveness and progression in epithelial ovarian neoplasm. The MTA1 could thus potentially provide information on the mechanism of cancer invasion and metastasis.
Subject(s)
Gene Expression , Immunohistochemistry , Neoplasm Metastasis , Ovarian NeoplasmsABSTRACT
OBJECTIVE: cDNA microarray and tissue array was utilized for the profiling of differentially expressed genes in uterine cervical squamous cell carcinoma. Metastasis associated 1 gene (MTA1) was investigated using these methods, and we correlated gene and protein expression of MTA1 with the invasion and metastasis of cancer. METHODS: Gene expression profiles for paired cancerous and noncancerous uterine cervical tissue samples from an individual by means of a cDNA microarray representing 17,000 genes were analyzed. Of the differentially expressed genes, we assessed the MTA1 gene at the protein level using tissue array and immunohistochemistry. RESULTS: The expressions of 15 and 21 genes were noted to have more than fivefold increase or decrease in the cervical squamous cell carcinoma tissue compared to the non-cancerous cervical tissue. The changed genes were those associated with DNA synthesis/repair, apoptosis, modulation of transcription, signal transduction, enzyme, cell cycle, cytoskeleton, metabolism, cell adhesion, extracellular matrix, immune response and others. Expression of MTA1 was evaluated by immunohistochemistry in 34 squamous cell carcinoma in situ, 32 microinvasive carcinoma and 56 invasive squamous cell carcinoma. Increased expression of MTA1 was significantly correlated with depth of invasion and lymph node metastasis. There was no statistically significant relationship between MTA1 expression and age, and FIGO stage. CONCLUSION: These results suggest that MTA1 may closely related to invasiveness and progression in cervical cancer. Thus, MTA1 could potentially provide information on the mechanism of cancer invasion and metastasis.
Subject(s)
Apoptosis , Carcinoma, Squamous Cell , Cell Adhesion , Cell Cycle , Cytoskeleton , DNA , DNA, Complementary , Extracellular Matrix , Gene Expression , Immunohistochemistry , Lymph Nodes , Metabolism , Neoplasm Metastasis , Oligonucleotide Array Sequence Analysis , Signal Transduction , Transcriptome , Uterine Cervical NeoplasmsABSTRACT
Cutaneous endometriosis seems to be more common in women who have had a pelvic or abdominal operation and primary cutaneous endometriosis is very rare. In our hospital we experienced a 41-year-old woman who complained of the appearance at the umbilicus of a nodule and had not had operation. This nodule was responsible for recurrent pain and increasing in size and it was excised. Pathology findings revealed cutaneous endometriosis. So we present a case of primary cutaneous endometriosis with a brief review of literature.
Subject(s)
Adult , Female , Humans , Endometriosis , Pathology , UmbilicusABSTRACT
Conjoined twins are rare congenital malformation that occurs one in 50,000-100,000 births. The site and fusion are variable. In recent years, prenatal diagnosis of conjoined twins with ultrasonogram (US), computed tomography (CT), and magnetic resonance imaging (MRI) has been reported. Early prenatal diagnosis and assessment of the degree of conjoining provided couples with the option for pregnancy termination via vaginal delivery. We report a case of cephalopagus diagnosed prenatally by ultrasonogram in the second trimester and subsequently which was terminated, with a brief review of literature.
Subject(s)
Female , Humans , Pregnancy , Family Characteristics , Magnetic Resonance Imaging , Parturition , Pregnancy Trimester, Second , Prenatal Diagnosis , Twins, Conjoined , UltrasonographyABSTRACT
OBJECTIVE: To assess the effect of recombinant human leukemia inhibitory factor on in vitro development of 1-cell ICR mouse embryo. MATERIALS AND METHOD: ICR mice were superovulated with PMSG/hCG and 1-cell stage mouse embryos were recruited. 1-cell mouse embryo were cocultured on human oviductal cells in a CO2 incubator (coculture group) and were cultured on 0.4% BSA+HTF media (control group). And anti-hLIF Ab was added the cocultured group in a different concentration (1pg, 10pg, 100pg, 1ng) and developmental rate was compaired to the control group, and rhLIF was added to the preincubated 0.4% BSA+HTF media in a different concentration (2000U, 1000U, 100U, 10U) and its developmental rate was compaired to group which was cultured on 0.4% BSA+HTF media only. RESULT: 1. The cleavage rate of 2-cell mouse embryo co-cultured with human tubal epithelial cell was significantly higher than that of cultured with media alone (HTF with 0.4% BSA) (p<0.05). 2. When LIF antibody was added to the medium with human tubal epitherlial cell, the mouse embryo could not cleave more than 2-cell in 1 ng of LIF antibody, and less than 1 ng, the cleavage rate was lower than cultured without LIF antibody group(p<0.05). 3. Two cell blocked ICR mouse embryos were developed into four cells under LIF(p<0.05), but no further development was observed. CONCLUSIONS: These results shows that LIF enhances the development of preimplantation embryo, and when rhLIF is applicated in vitro, it has positive effects on the development of early mouse embryo and can help overcoming the two-cell block.